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. Author manuscript; available in PMC: 2014 Mar 17.
Published in final edited form as: Sci Transl Med. 2012 Jan 18;4(117):117ra9. doi: 10.1126/scitranslmed.3003041

Fig. 2.

Fig. 2

IL-17A is required for smoke-induced emphysema in mice. (A) Numbers of total macrophages (Mac), lymphocytes (Lym), and neutrophils (Neu) in BALF of air- and cigarette smoke–exposed wild-type (WT) and Cc10- Il17a mice (n = 5 per group). (B) Numbers of cells as in (A) in BALF from WT and Il17a−/− mice (n = 4 to 8 per group). *P < 0.05; **P < 0.01; ***P < 0.001. (C) Representative H&E staining of lung sections from air- or cigarette smoke–exposed mice as indicated on each panel; inset represents ×200 magnification. Scale bars, 100 μm. (D and E) μCT quantification of total lung volume and lung density from air (–)– and cigarette smoke (+)–exposed WT and Cc10-Il17a mice (D) or WT and Il17a−/− mice (E) (n = 4 to 8 per group). *P < 0.05; **P < 0.01; ***P < 0.001. Data represent at least three independent studies. (F) Intracellular cytokine staining of IL-17A and IFN-γ from lung CD4+ T cells of air- or smoke-exposed WT and Cc10-Il17a mice. Data represent at least three independent studies with five mice in each group. (G) Mmp12 mRNA expression from total BALF cells of airand smoke-exposed mice (n = 4 to 8 per group). **P < 0.01. (H) Numbers of cells as in (A) in BALF from WT and Tcrδ−/− mice (n = 4 to 5 per group). *P < 0.05; **P < 0.01; ***P < 0.001. (I) Intracellular cytokine staining of IL-17A and IFN-γ from lung CD4+ T cells of air- or smoke-exposed WT and Tcrδ−/− mice. Data represent at least three independent studies with four to five mice in each group. (J) μCT quantification of total lung volume and lung density from air- and cigarette smoke–exposed WT and Tcrδ−/− mice (n = 4 to 5 per group). *P < 0.05; **P < 0.01. (K) Mmp12 mRNA expression from total BALF cells of air- and smoke-exposed WT and Tcrδ−/− mice (n = 4 to 8 per group). *P < 0.01.