Skip to main content
. Author manuscript; available in PMC: 2014 Mar 17.
Published in final edited form as: Sci Transl Med. 2012 Jan 18;4(117):117ra9. doi: 10.1126/scitranslmed.3003041

Fig. 6.

Fig. 6

Irf7 regulates the function of Spp1−/− lung APCs. (A) Heat map depicting significantly up-regulated type I IFN–stimulated genes (fold change > 1.5) from gene microarray analysis of mouse lung APCs of cigarette smoke–exposed WT (n = 2) and Spp1−/− (n = 2). (B) Irf7 mRNA expression in total BALF cells from air- and smoke-exposed WT and Spp1−/− mice (n = 5 per group). ***P < 0.001. (C to E) Il6, Il1b, and Tgfb1 mRNA expression in lung APCs from air- and smoke-exposed WT and Spp1−/− mice (n = 3 per group). **P < 0.01; ***P < 0.001. ns, nonsignificant. (F) Irf7, Il6, Il1b, and Mmp12 mRNA expression in lung APCs from smoke-exposed Spp1−/− mice and transfected with scrambled or Irf7 siRNA (n = 6 per group). *P < 0.05; **P < 0.01. (G) IL-17A and IFN-γ in spleen-derived CD4+ T cells cocultured with lung APCs as described in (F) for 3 days. Protein production was measured by Milliplex kit (n = 5 independent experiments with triplicate replicates in each experiment). **P < 0.01.