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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Cell. 2013 Dec 19;156(0):183–194. doi: 10.1016/j.cell.2013.11.028

Figure 3. Effect of metabolism on cytoplasmic motion depends on particle size.

Figure 3

(A) MSD of GFP-μNS particles of varying binned fluorescence intensities in E. coli cells (CJW4617) under normal growth conditions (M2G).

(B) Same as A, but for GFP-μNS particles in E. coli cells (CJW4617) under DNP treatment.

(C) Histograms of GFP-μNS particle displacements under untreated and DNP-treated conditions for selected bins. Displacements were measured over 15-sec intervals. Line width indicates Poisson counting error, and the gray shading delineates the estimated tracking error.

(D) Mean radius of gyration (Rg) of all trajectories from GFP-μNS particles of a given binned size is plotted as a measure of spatial exploration for untreated and DNP-treated cells.

(E) The ratio (RgUntreated-Rg+DNP)/Rg+DNP is plotted as a function of particle size.

See also Figs. S6–7.