Table 2.
Toxicity, resistance levels, and synergy in Culex quinquefasciatus tested with Mtx-2 combined with various Cry toxins from Bacillus thuringiensis at a 1:1 ratio.
| Toxin(s) | Colony | LC50 (FL) (μg/ml) | LC95 (FL) (μg/ml) | Resistance ratio
|
Synergy factor
|
||
|---|---|---|---|---|---|---|---|
| LC50 | LC95 | LC50 | LC95 | ||||
| Mtx-2 | CqSyn | 4.13 (2.37–7.20) | 347.9 (102–1216) | 1.0 | 1.0 | ||
| Cq11A | 49.6 (31.9–77.6) | 702.7 (251.4–2053) | 12.0 | 2.0 | |||
| CqAB | 22.4 (18.3–27.5) | 500 (326–867) | 5.4 | 1.4 | |||
| CqAB11A | 97.9 (78.3–128) | 1696 (951–3869) | 23.7 | 4.9 | |||
| CqAB11Acyt | 107 (59.6–194) | 696 (139–3736) | 25.9 | 2.0 | |||
| Cry11Aa | CqSyn | 1.30 (0.792–2.12) | 14.2 (5.73–35.6) | 1.0 | 1.0 | ||
| Cq11A | Average mortality 47% at 200 μg/ml | 154 | na | ||||
| Cry4Aa | CqSyn | 5.04 (1.89–13.4) | 983 (52.5–19219) | 1.0 | 1.0 | ||
| CqAB | Average mortality 14% at 200 μg/ml | >40 | na | ||||
| Cry4Ba | CqSyn | Average 6% mortality at 200 μg/ml | |||||
| CqAB | Average 1% mortality at 200 μg/ml | ||||||
| Cry4A, Cry4B | CqSyn | 1.49 (0.961–2.30) | 15.2 (6.78–34.5) | 1.0 | 1.0 | ||
| CqAB | 315 (188–722) | 35481 (8245–442709) | 211 | 2334 | |||
| Cry4A, Cry4B, Cry11A | CqSyn | 0.00820 (0.00586–0.0115) | 0.0549 (0.0297–0.105) | 1.0 | 1.0 | ||
| CqAB11A | 0.590 (0.409–0.849) | 11.7 (5.78–24.5) | 80.0 | 213 | |||
| Cry4A, Cry4B, Cry11A, Cyt1A | CqSyn | 0.020 (0.0174) | 0.0229 | 1.0 | 1.0 | ||
| CqAB11Acyt | 0.0753 (0.0648–0.0877) | 0.582 (0.437–0.837) | 3.8 | 5.4 | |||
| Mtx-2, Cry11A | CqSyn | 0.904 (0.447–1.83) | 40.8 (9.97–169) | 1.0 | 1.0 | 2.2 | 0.67 |
| Cq11A | Average mortality 53.8% at 200 μg/ml | 222 | na | none | |||
| Mtx-2, Cry4A | CqSyn | 0.268 (0.200–0.357) | 1.31 (0.783–2.28) | 1.0 | 1.0 | 16.8 | 392 |
| Cq4AB | 1.21 (0.781–1.87) | 8.18 (3.04–24.1) | 4.5 | 6.2 | 37.0 | 122 | |
| Mtx-2, Cry4B | CqSyn | 85.7 (72.0–104) | 673 (448–1190) | 1.0 | 1.0 | 0.1 | 0.5 |
| CqAB | Average mortality 29% at 200 μg/ml | na | na | na | na | ||
| Mtx-2, Cry4A, Cry4B | CqSyn | 0.114 (0.0997–0.130) | 0.567 (0.454–0.750) | 1.0 | 1.0 | 19.2 | 51.3 |
| CqAB | 0.324 (0.281–0.371) | 1.73 (1.39–2.29) | 2.8 | 3.1 | 129 | 570 | |
| Mtx-2, Cry4A, Cry4B, Cry11A | CqSyn | 0.0295 (0.0261–0.0332) | 0.105 (0.0875–0.134) | 1.0 | 1.0 | 0.6 | 1.0 |
| CqAB11A | 0.0564 (0.0505–0.0629) | 0.182 (0.153–0.228) | 1.9 | 1.7 | 20.7 | 127.5 | |
| Mtx-2, Cry4A, Cry4B, Cry11A, Cyt1A | CqSyn | 0.0517 (0.0450–0.0592) | 0.298 (0.237–0.395) | 1.0 | 1.0 | 0.77 | 0.72 |
| CqAB11Acyt | 0.163 (0.141–0.189) | 1.09 (0.854–1.46) | 3.2 | 3.7 | 0.9 | 1.1 | |