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. 2014 Mar 17;9(3):e91524. doi: 10.1371/journal.pone.0091524

Figure 1. Gene structure of sll1393 (A) and sll0945 (B) on Synechocystis sp. PCC 6803 genomic DNA.

Figure 1

The targeted replacement of each GS gene with the KmR gene was achieved by homologous recombination. (C) PCR analysis of sll1393 and sll0945 genes using genomic DNA from wild-type, sll1393−, and sll0945− strains as templates. For comparison, PCR products of intact genes in wild-type were loaded next (left lane) to the corresponding mutated genes. Lane1 is the size marker (1 kb plus DNA ladder, Life Technologies). DNA bands on lane 2 (WT) and 3 (M1) were amplified using prA1 and prA4; Lane 4 (WT) and 5 (M2) were amplified using prB1 and prB4.