Table 3.
Oligos Used for the Construction of Gene Libraries 1 and 2
Library 1 Forward | 5′-CTAGCTGT CAT ATG TCT AAC AAA GGC GCG ANT ANT GNT CTG ATG GNT GNT GNT GNT GNT ATT GCG GAT AGT CAT AGT TAA-3′ |
Library 2 Forward | 5′-CTAGCTGT CAT ATG CAG AAA CTG GNT DTN DTN GCG GAA GNT GNT GGC TCT AAC AAA TAA ATT GCG GAT AGT CAT AGT TAA-3′ |
Reverse | 5′-CGATAGGAC GAATTC CAG TGG TAG CTT GTG TGC CAA CAG TAG TTA ACG GTA GAA CCG TTA ACT ATG ACT ATC CGC AAT -3′ |
Degenerate codons are underlined. Complementary regions are colored blue. All oligos are written from 5′-3′. N: equal mixture of A, T, G and C. D: Equal mixture of A, G and T.