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. 2014 Mar 17;9(3):e91351. doi: 10.1371/journal.pone.0091351

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© 2014 Barresi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) OPHN1 mRNA expression (left panel) and editing levels at ADAR2-specific sites (right panel) in U118 ADAR2 E/A (light grey) and U118 ADAR2 (dark grey) cells. The expression levels were calculated as a relative-fold increase compared to inactive ADAR2 (E/A) arbitrarily set to 1. Each sample was normalized to β-actin mRNA levels. Mean ± s.d. (n = 3), ** p<0.01. (B) The same experiments showed in (A) were performed in the U87 cell line. (C) OPHN1 mRNA expression (left panel) and editing levels at the ADAR1-specific sites (right panel) in U118 scramble (light grey) and U118 siADAR1 (dark grey) cells. The expression levels of the samples were calculated as a relative-fold increase compared to the scramble arbitrarily set to 1. Each sample was normalized to β-actin mRNA levels. Mean ± s.d. (n = 3). (D) The same experiments showed in (C) were performed in the U87 cell line.