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. 2014 Mar 11;9:1311–1322. doi: 10.2147/IJN.S59726

Figure 8.

Figure 8

(A) Green fluorescent protein reporter gene transfection in HeLa cells by OTMCS-PEI-R13 at w/w ratios of 5, 10, 20, and 30.* Percentage of green fluorescent protein transfection in HeLa cells by flow cytometry analysis. (B) Representative fluorescence images for the transfection of HeLa cells using OTMCS-PEI-R13 at w/w ratios of 5, 10, 20, and 30.* (C) Transfection efficiency of OTMCS-PEI-R13/DNA complexes in an HeLa cell line at w/w ratios of 5, 10, 20, and 30.* (D) Transfection efficiency of pGL3-control as a reporter gene in mice.

Notes: BALB/c athymic mice were inoculated with HeLa cells. Luciferase gene expression was determined after administration of the OTMCS-PEI-R13/DNA complex (w/w =10), OTMCS-PEI-R13/DNA complex (w/w =30), OTMCS-PEI and PEI 25 kDa/DNA complex (N/P =5); *Each data point represents the mean ± standard deviation of n=5. **P<0.01. The results are expressed in relative light units/mg protein.

Abbreviations: OTMCS, N-octyl-N-quaternary chitosan; PEI, polyethylenimine; R13, RGDC-TAT (49–57); GFP, green fluorescent protein; RLU, relative light units; w/w, weight/weight.