Fig 5.

Analysis of inflammatory gene and protein expression in tongue tissues.A. Two chemokines (CXCL1, CXCL2), a neutrophil-specific antigen (CD177) and an epithelial cell-derived neutrophil-activating cytokine (IL-17C) were tested in the same RNA samples surveyed by microarray analysis on day 5 post infection. After cDNA synthesis, equal amounts from three mice per group were mixed and analysed in triplicate by RT-qPCR. All genes were significantly (P < 0.05) upregulated in co-infected animals (CaSo) compared with C. albicans alone (Ca).B. MIP-2/CXCL2 protein levels (day 5) in tongue homogenates, normalized by tissue weight (pg g⁻¹ of tissue). MIP-2 was not detectable in uninfected mice (not shown). Results are means from three independent animal experiments, with 4–6 animals per group and error bars represent SEM. *P < 0.01 compared with C. albicans alone.