Fig 6.

Increased neutrophil infiltration of the oral mucosa in co-infected mice.A. Immunofluorescence staining of frozen tongue sections (day 5) with monoclonal antibody NIMP-R14, highly specific for murine Ly-6G and Ly-6C. Neutrophils (red) are shown with white arrows. Note the intense staining in direct juxtaposition to the mixed bacterial-fungal biofilm in co-infected animals. Isotype control antibody did not show any staining (not shown). Bars = 50 μm.B. MPO activity in tongue homogenates on day 5 post infection. Results are means from two independent animal experiments, with 8–10 animals per group and error bars represent SEM. *P < 0.05 compared with C. albicans alone.