Fig 7.
HEK293-hTLR2 expressing cells respond to S. oralis but not C. albicans with IL-8 secretion. HEK293-hTLR2 cells were challenged with live S. oralis 34 (So) and/or C. albicans (Ca) and supernatants were analysed by ELISA. Pam2CSK4 was used as a positive control. Two microbial cell doses were tested (Ca: 104, 105 cells ml−1, So: 105, 106 cells ml−1). IL-8 was undetectable with all stimulants in the HEK293-pcDNA3 negative control cells (not shown). Error bars represent SD of triplicate experiments. *P < 0.01 compared with medium only.