Fig 9.

TLR2 is upregulated in the oral mucosa during co-infection.A. TLR2 transcripts were analysed in tongue homogenates on day 5 by qRT-PCR. Results represent mean fold expression levels over uninfected animals in each of the four infection groups (C. albicans alone (Ca), S. oralis alone (So), high dose of S. oralis alone (H-So) or co-infected with C. albicans and S. oralis (CaSo)) with 4–6 animals per group. Error bars are SD. *P < 0.005, compared with Ca group.B. TLR2 protein expression levels on day 5 were analysed in tongue homogenates of wild type (WT) animals by Western blotting. A representative Western is shown with tissue samples from one animal in each of the four infection groups: So: S. oralis alone, High So: high dose of S. oralis, Ca: C. albicans alone and CaSo: co-infected with C. albicans and S. oralis. GAPDH signal served as internal loading control.