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. 2014 Mar 17;9(3):e91747. doi: 10.1371/journal.pone.0091747

Figure 4. LSR expression in a claudin-low breast cancer cell line.

Figure 4

Hs578t cells were stably transfected with either a control plasmid (pCMV), or a plasmid containing the full-length gene for LSR variant 1 (LSR+). Cell lines were grown to 80% confluence; lysates were isolated and analyzed via western analysis using and LSR specific antibody and α-tubulin for loading control. Intensity measured via ImageJ. (A) Representative western blots. (B) Data represent mean relative intensity +/− SE. *P<0.001. (C) Representative images of cells growing on tissue culture treated dishes (left panels; 20× magnification) and cells subjected to immunocytofluorescence using a LSR specific antibody (right panels; DNA stained with DAPI, 40× magnification). (D) Proliferation assays. Cells were plated at 50,000 cells per well in triplicate and counted every 24 h for 96 h. Data represent mean +/− SE. *P<0.001. (E) Cell cycle analysis: cells growing in log-phase were fixed and stained with propidium iodine and analyzed via flow cytometry. Data represent mean +/− SE. *P<0.03.