O-GlcNAcylation in developing mouse brains and mouse NSCs. A: Expression of O-GlcNAcT and O-GlcNAcase in developing mouse brains [embryonic day (E) 12, E14, E16, E18, postnatal day (P) 1, P10] and adult mouse brains (A) was examined by RTPCR. B: Expression of O-GlcNAcT and O-GlcNAcase in adult mouse brains, E14 brains, neurospheres (floating cell aggregates formed by NSCs in vitro) and NECs was examined. Sox2 is a marker gene of undifferentiated NSCs. “–RT” indicates negative controls without reverse transcription. C: Expression patterns of O-GlcNAcylated proteins in adult mouse brains, E14 brains, neurospheres, and NECs were analyzed by Western blot. A mouse IgM (coIgM) was used as a subclass control for anti-O-GlcNAc antibody. Nestin is a marker protein of undifferentiated NSCs.