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. Author manuscript; available in PMC: 2014 Mar 18.
Published in final edited form as: J Neurosci Res. 2009 Dec;87(16):3535–3545. doi: 10.1002/jnr.22170

Fig. 2.

Fig. 2

Effects of the O-GlcNAcase inhibitor, PUGNAc, on NECs. A:O-GlcNAcylation in NECs treated with or without PUGNAc (100 μM) for 24 hr was examined by immunocytochemistry. Nuclei and O-GlcNAc were stained with Hoechst 33258 and CTC110.6 anti-O-GlcNAc antibody, respectively. B: Dose-dependent increases of O-GlcNAcylation in NECs treated with PUGNAc for 24 hr was examined by Western blot analysis. C: Correlation between the NEC number and the spectrophotometric absorbance (Abs) of WST-8-formazan produced by living NECs. The absorbance was measured at the wavelength of 450 nm (reference, 650 nm). D: The NEC number reduction by treatment with PUGNAc for 0–4 days was traced by the WST-8 assay. bFGF was used as a mitogen.