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. Author manuscript; available in PMC: 2014 Mar 18.
Published in final edited form as: J Neurosci Res. 2009 Dec;87(16):3535–3545. doi: 10.1002/jnr.22170

Fig. 6.

Fig. 6

Proteasome activity in NECs treated with O-GlcNAcase inhibitors. Lysates of NECs treated with or without Stz for 8 hr (A) or PUGNAc for 24 hr (B) were incubated with a fluorogenic substrate of the proteasome succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide. After 4 hr, the fluorescence intensity of 4-methylcoumaryl-7-amide cleaved from the substrate by the proteasome activity was measured by using a multilabel plate reader equipped with a 360-nm excitation filter and a 460-nm emission filter. Epigallocatechin gallate (EGCG), a proteasome inhibitor, was used as a negative control.