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. Author manuscript; available in PMC: 2014 Mar 18.
Published in final edited form as: J Neurosci Res. 2009 Dec;87(16):3535–3545. doi: 10.1002/jnr.22170

Fig. 8.

Fig. 8

Identification of Sp1 transcription factor as an O-GlcNAcylated protein. A: Expression of thymidine kinase, a target gene of Sp1, in NECs treated with PUGNAc (100 μM) for 24 hr was analyzed by RT-PCR. “–RT” indicates negative controls without reverse transcription. B: Lysates prepared from PUGNAc-treated NECs were immunoprecipitated with anti-Sp1 polyclonal antibody and protein A-Sepharose. The immunoprecipitates (IP) were analyzed by Western blot with anti-Sp1 antibody and then reprobed with anti-O-GlcNAc antibody.