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. 2014 Mar 17;9(3):e91709. doi: 10.1371/journal.pone.0091709

Figure 3. The sensitivity of breast cancer cell lines to TGF-β.

Figure 3

(A) Induction of PAI-1 mRNA by TGF-β in breast cancer cell lines. MDA-MB-231 and SK-BR-3 cells were treated for 2 h with or without TGF-β (100 pM) before isolation of total RNA with Trizol. PAI-1 mRNA levels were determined with RT-Quantitative PCR. Data are means±SD of at least three independent experiments in each condition. * P<0.05. (B) TGF-β induced phosphorylation of Smad2 in SK-BR-3 cells. SK-BR-3 cells in complete medium was treated for 30 min with or without TGF-β(100pM) before being lysed with SDS sample buffer. The level of Phospho-Smad2 was analyzed by immunoblotting.