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. 2014 Mar 17;9(3):e91644. doi: 10.1371/journal.pone.0091644

Figure 1. RRM2 protein expressions in the SiHa cancer cells of the uterine cervix with shRRM2 #354 or #962 and their correlations with cell viability.

Figure 1

(A) Protein levels of RRM2 were detected in the SiHa cervical cancer cells with shRRM2 #354 or #962 using Western blotting. β-actin was used as the internal control. The relative expressions of RRM2/β-actin are showed. The effect of RRM2 gene knockdown was stronger in the SiHa cells with shRRM2 #354 compared to those with shRRM2 #962. (B) A greater toxic effect on cell viability using cisplatin was found in the SiHa cells with shRRM2 #354 than in those with shRRM2 #962 compared to the control SiHa shLuc cells, in which shLuc was transfected into SiHa cells. Cell viabilities were detected using 3-(4,5-cimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide assay. RRM2, ribonucleotide reductase M2; ***p<0.001.