Table 1. Plasmids used in this work.
Name | Backbone | Characteristics | Reference |
pDR113 | pMLB1115 | PLac::minE-gfpmut2 | [27] |
pDR119 | pMLB1113 | PLac::gfpmut2-minD | [18] |
pDSW208-Z338-YFP | pDSW208 | ftsZ'::eyfp::'ftsZ (YFP inserted between the 338 and 339 FtsZ residues) | [49] |
pDSW208/210 | pTrc99A | AmpR, Promoter down mutations in -35 and -10 of pTrc99A | [38] |
pET-FtsZ-mts | pET11-b | PT7::ftsZ(Δ1099-1152 bp)::venus::minD(Δ1-762 bp) | [21] |
pKG110 | pLC112 | CamR, Pnah | [50] |
pPZV42 | pTrc99A | PTrc:: ftsZ(Δ1099-1152 bp)::venus::minD(Δ1-762 bp) | This work |
pPZV46 | pET28-a | PT7::his6-minE | This work |
pPZV65 | pKG110 | yfp::ftsZ | This work |
pPZV110 | pDSW210 | yfp::ftsZ | This work |
pPZV137 | pDSW210 | yfp::ftsZ(Δ1099-1152 bp) | This work |
pPZV138 | pDSW210 | ftsZ | This work |
pTrc99A | N/A | PTrc, ampR | [37] |