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. 2014 Mar 17;9(3):e91955. doi: 10.1371/journal.pone.0091955

Figure 1. Inhibition of type-I collagen and fibronectin expression in dermal fibroblasts by kynurenines.

Figure 1

Type-I Collagen and fibronectin expression at the mRNA and protein level in cultured fibroblasts treated with increasing concentrations (6.25, 12.5, 25, 50, 100, and 150 μg/ml) of KynA, Kyn, L-Kyn or D-Kyn. A & B: Relative type-I collagen and fibronectin mRNA expression in treated fibroblasts, respectively. GAPDH was used as the reference gene. C: Evaluation of type-I collagen and fibronectin expression at the protein level using Western blotting. D & E: The Mean±SEM ratio of type-I collagen and fibronectin density to β-actin at the protein level, respectively. β-actin was used as protein loading control.