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A: Evaluation of MMP3 mRNA expression in fibroblasts treated with increasing doses (6.25, 12.5, 25, 50, 100, and 150 μg/ml) of KynA, Kyn, L-Kyn or D-Kyn following 24 hours of treatment. GAPDH was used as loading control for Q-PCR. B: Evaluation of MMP3 presence in the fibroblast conditioned medium using Western blotting after 48 hours of treatment. C: The Mean±SEM ratio of MMP3 density at the protein level.
