Table 1. Thermodynamic and dissociation constants for ERK2:KIM-PTP and DUSP16 MKB domains derived from ITC experiments at 25 °C.

Complex	Kd (nM)	ΔH (kcal·mol−1)	TΔS (kcal·mol−1)
ERK2: STEPKIM	546±56	−27.9±0.4	−19.4±0.3
ERK2: STEPKIMKIS	123±28	−29.2±0.8	−19.7±0.9
ERK2: STEP	48±20	−29.5±2.5	−19.7±2.0
ERK2: PTPSLKIM	463±24	−23.5±1.1	−14.9±1.1
ERK2: PTPSLKIMKIS	825±190	−21.6±0.8	−13.3±0.9
ERK2: PTPSL	108±36	−22.3±2.2	−12.8±2.1
ERK2: PSchimera	47±6	−18.5±0.8	−8.5±0.7
ERK2: DUSP16 MKBD	1303±215	−4.8±0.2	−3.2±0.3

All experiments were performed in triplicate and values reported are the experimental average and standard deviation.