Skip to main content
. 2014 Mar 17;9(3):e92054. doi: 10.1371/journal.pone.0092054

Figure 9. Different effects of Blk-haploinsufficiency and Blk-deficiency on in vivo γδ-17 effector function.

Figure 9

(A) Graph comparing numbers of γδ-17 and DN-17 cells from B6 (n = 8), Blk+/− (n = 7), and Blk−/− (n = 6) mice. (B) B6, Blk+/− and Blk−/− mice were infected with Listeria monocytogenes. 5 days later, splenocytes were harvested and γδ-17 and DN-17 cells were in vitro stimulated for 4 hours with a cocktail of IL-23, IL-1, and Pam3Cys in the presence of brefeldin A. Dot plots showing CD3 versus IL-17A expression in gated γδ T cells from each of the three genotypes. Adjacent graph compares percentage of IL-17A+ per T cell subset in B6 (n = 10), Blk+/− (n = 6), and Blk−/− (n = 6) mice. *p ≤ 0.05; **p ≤ 0.01; #p ≤ 0.001.