Table 1. Comparison of immune cell numbers in 3-month-old B6, Blk+/−, B6.lpr and Blk+/−.lpr mice.
| B6 | Blk+/− | p value | B6.lpr | Blk+/−.lpr | p value | |
| Spleen | 89.1 ± 4.0a | 114.3 ± 6.8 | 0.002 | 124.7 ± 7.2 | 155.4 ± 9.2 | 0.01 |
| Total B cells (CD19+) | 40.8 ± 2.3 | 49.3 ± 3.8 | 0.05 | 62 ± 3.1 | 70.7 ± 4.0 | 0.1 |
| Transitional (CD93+CD19+) | 5.5 ± 0.5 | 6.1 ± 1.0 | 0.6 | 14.3 ± 1.2 | 15.8 ± 1.6 | 0.5 |
| FO B cells (CD93−CD23hiCD21lo) | 32 ± 2.1 | 36.5 ± 2.8 | 0.2 | 36.6 ± 2.2 | 43.2 ± 3.1 | 0.1 |
| MZ B cells (CD93−CD23l°CD21hi) | 2.9 ± 0.3 | 2.4 ± 0.3 | 0.2 | 4.0 ± 0.3 | 3.9 ± 0.4 | 0.9 |
| Pre-plasmablasts (CD93−CD23l°CD21lo) | 1.7 ± 0.2 | 3.1 ± 0.4 | 0.003 | 4.6 ± 0.3 | 5.9 ± 0.5 | 0.03 |
| B1 B cells (CD5+IgM+) | 0.7 ± 0.09 | 1.3 ± 0.1 | 0.002 | 1.5 ± 0.1 | 1.8 ± .09 | 0.1 |
| Total T cells (CD3+) | 33.1 ± 2.4 | 48.5 ± 3.4 | 0.0007 | 41.2 ± 5.5 | 58.0 ± 5.0 | 0.03 |
| Total CD4+ cells | 18.6 ± 1.5 | 26.8 ± 2.1 | 0.002 | 20.1 ± 2.4 | 27.8 ± 2.2 | 0.02 |
| Treg cells (CD4+Foxp3+) | 3.4 ± 0.3 | 3.8 ± 0.3 | 0.4 | 5.8 ± 0.7 | 7.2 ± 0.5 | 0.1 |
| Total CD8+ cells | 12.8 ± 0.9 | 18.2 ± 1.1 | 0.0006 | 11.3 ± 1.9 | 13.9 ± 1.2 | 0.2 |
| Total DN αβ T cells | 0.9 ± 0.07 | 1.5 ± 0.2 | 0.0006 | 8.2 ± 1.3 | 14.1 ± 1.7 | 0.02 |
| B220+ DN αβ T cells | 0.2 ± 0.02 | 0.4 ± 0.06 | 0.01 | 6.4 ± 1.1 | 11.7 ± 1.6 | 0.02 |
| γδ T cells | 1.1 ± 0.08 | 1.9 ± 0.2 | 0.0009 | 1.4 ± 0.2 | 1.9 ± 0.2 | 0.07 |
| Macrophages (CD19−F4/80+) | 1.8 ± 0.2 | 2.5 ± 0.3 | 0.05 | 5.1 ± 0.6 | 5.3 ± 0.5 | 0.8 |
| cDCs (CD19−CD11c+CD317−) | 0.8 ± 0.07 | 1.2 ± 0.1 | 0.08 | 1.7 ± 0.1 | 1.8 ± 0.1 | 0.6 |
| pDCs (CD19−CD11c+CD317+) | 0.13 ± 0.01 | 0.22 ± 0.04 | 0.06 | 0.18 ± 0.01 | 0.24 ± 0.02 | 0.03 |
| Peritoneal Cavity | 1.4 ± 0.2 | 2.3 ± 0.5 | 0.08 | 3.6 ± 0.4 | 3.0 ± 0.4 | 0.3 |
| B1 B cells (CD5+IgM+) | 0.019 ± 0.005 | 0.023 ± 0.006 | 0.5 | 0.3 ± 0.007 | 0.4 ± 0.006 | 0.7 |
| Lymph Nodes | 26.4 ± 1.1 | 29.5 ± 1.5 | 0.1 | 54.0 ± 7.9 | 66.1 ± 9.3 | 0.3 |
| Total T cells (CD3+) | 18.8 ± 1.1 | 22.2 ± 1.4 | 0.06 | 33.2 ± 5.7 | 40.1 ± 6.3 | 0.4 |
| Total CD4+ cells | 9.5 ± 0.6 | 11.2 ± 0.7 | 0.09 | 9.3 ± 1.3 | 11.7 ± 1.8 | 0.3 |
| Total CD8+ cells | 8.7 ± 0.5 | 10.1 ± 0.6 | 0.06 | 10.4 ± 1.4 | 12.8 ± 2.2 | 0.4 |
| Total DN αβ T cells | 0.2 ± 0.02 | 0.3 ± 0.05 | 0.06 | 12.0 ± 3.3 | 14.0 ± 3.1 | 0.7 |
| B220+ DN αβ T cells | 0.04 ± 0.009 | 0.06 ± 0.01 | 0.2 | 10.7 ± 3.0 | 12.4 ± 2.9 | 0.7 |
| γδ T cells | 0.3 ± 0.02 | 0.4 ± 0.03 | 0.006 | 0.8 ± 0.1 | 1.3 ± 0.3 | 0.09 |
a
Mean number of cells per tissue or subset ± SEM × 106. n = 10 to 27 mice per genotype.