Table 1. Factors and levels in the response surface design.
| Factor | Symbol | Levels | ||
| −1 | 0 | 1 | ||
| Enzyme amount (U/g) | x1 | 2000 | 5000 | 8000 |
| Substrate concentration (g/ml) | x2 | 0.02 | 0.04 | 0.06 |
| Time (h) | x3 | 2 | 4 | 6 |
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