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. 2014 Mar 17;9(3):e92242. doi: 10.1371/journal.pone.0092242

Figure 2. Fasudil reduces the production of various inflammatory cytokines.

Figure 2

Serum samples were detected using an enzyme-linked immunosorbent assay at 12 h after ischemia/reperfusion (I/R). Interleukin (IL)-6 (A), chemokine (C-C motif) ligand 2 (CCL2) (B), tumor necrosis factor (TNF)-α (C) and IL-10 (D). Fasudil also reduces the ischemia/reperfusion (I/R)-induced expression of proinflammatory cytokines in leukocytes. Total RNA of leukocytes was collected at 12 h after I/R and detected using real-time polymerase chain reaction. The mRNA expression levels of IL-6 (E), CCL2 (F), TNF-α (G), and IL-10 (H) were assessed. Data represent means from at least 5 mice each. * p<0.05, ** p<0.01.