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. 2014 Mar 17;9(3):e92242. doi: 10.1371/journal.pone.0092242

Figure 3. Fasudil reduces neutrophil infiltration after I/R.

Figure 3

Immunohistochemical analysis of neutrophils stained with anti-Gr-1 are shown (A). Myeloperoxidase (MPO) activity at 6 h after ischemia/reperfusion (I/R) (B). Relative rate of accumulation of leukocytes and neutrophils during I/R (C). Representative FACS data, in which anti-CD45 or the isotype control was used to stain cells isolated from the sham, I/R, or I/R+F groups 12 h after I/R (D). Quantitative measurements of CD45+ cell number in the sham, I/R plus vehicle (I/R+V), and I/R plus fasudil (I/R+F) groups (E). Representative FACS data for CD45+ cells purified using immunobeads and stained with anti-Gr-1+ (F). Summary data of FACS analysis. The results of quantitative analysis of the number of Gr-1+ cells among CD45+ cells are shown (G). Data represent means from at least 4 mice each. * p<0.05, ** p<0.01. Fasudil attenuates chemotaxis and cytokine production of leukocytes in vitro. Chemotaxis and production of IL-6 were significantly reduced in the presence of fasudil (10μM) (H and I).