Table 1.
Primers used in RT-PCR
| Gene Name | Primer Sequence | Annealing Temperature |
|---|---|---|
| Osteocalcin |
Forward: GGC AAT AAG CTA GTG AAC AG Revers: GGT CCT AAA TAG TGA TAC CGT |
60 |
| Runx2 |
Forward: CAG CAT CCT ATC AGT TCC CAA Revers: CAG CGT CAA CAC CAT CAT |
60 |
| PPARgamma |
Forward: GAG CAC TTC ACA AGA AAT TAC C Revers: AAT GCT GGA GAA ATC AAC TG |
60 |
| LPL |
Forward :AAT TGT CCC ATG CTG TAA CC Revers:CAG GAC ACA GGA AGC TAA GG |
60 |
| CollagenII |
Forward: GTTCACATACACTGCCCT Revers: GTCCACACCAAATTCCTC |
60 |
| Aggrecan |
Forward: CCCAGAGAAATTCACTTCC Revers :TAGATAGACAGTCCTTACACCC |
60 |
| Sox9 |
Forward: CCAGCGTTTAACCTTCAAGAC Revers: ATTTAACAACAGATGACCATACCC |
60 |
| -cateninβ |
Forward: AGAACACTAATTCATAATCACGCT Revers: GGCTCAAATAACACCTCTTACTG |
60 |
| TCF |
Forward: TACCCTACAAATGCTTCTCCTG Revers: AAACGTATCCTAGTCCCTCCT |
60 |
| GAPDH |
Forward: CAA CTC CCA CTC TTC CAC TT Revers: GCAGCGAACTTTATTGATGGTA |
60 |