Fig. 4.

Predicting the specificity of similar bHLH TFs. (A1) Predicted PWMs for MyoD (left) and NeuroD2 (right), (A2) Discriminative motifs based on direct comparison of MyoD sites (foreground) with NeuroD2 sites (background), suggesting MyoD and NeuroD2 preferred ebox and cofactor motifs. (B1 and B2) Comparison of MyoD and MSC. Similar to A1 and A2. (B3) Gel shift demonstrating that MSC/E12 heterodimer binds strongly at CCAGCTGG and MyoD/E12 binds weakly, whereas GCAGCTGC binds strongly to both MyoD/E12 and MSC/E12. MSC homodimer also binds stronger at CCAGCTGG than GCAGCTGC