Table 3. Comparison of NIF with various sandwich ELISA procedures and the commercial kits for the detection of HFA.
| Manufacturer/Immunoassay Procedures | Antibody binding | Sensitivity (ng mL−1) | References |
|---|---|---|---|
| New ELISA format (NIF) | One-step | 0.01 | This work |
| ELISA-covalent binding of antibody | Covalent | 0.03 | This work5 using the covalent immobilization of the antibody as described by Dixit et al.5 |
| Conventional ELISA | Passively adsorbed | 0.5 | This work |
| RnD systems | Passively adsorbed | 0.37 | http://www.rndsystems.com/pdf/DY1184.pdf |
| Biovendor | Passively adsorbed | 0.35 | http://www.biovendor.com/product/immunoassays/fetuin-a-ahsg-human-elisa |
| Alpco Diagnostics | Passively adsorbed | 5.00 | http://www.alpco.com/pdfs/43/43-NSEHU-E01.pdf |
| Immunology Consultants Laboratory, Inc. | Passively adsorbed | 6.25 | http://www.life-sciences.com.br/pdf/icllab.pdf |
| Genway Biotech, Inc. | Passively adsorbed | 6.25 | http://www.genwaybio.com/images/gw_tds/elisa_kits/40-374-130036.pdf |
| Assay Pro | Passively adsorbed | 6.25 | http://www.assaypro.com/datasheet/eg3501_1.pdf |