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. 2014 Jan 8;53(3):601–609. doi: 10.1021/bi400931k

Table 3. Activities of the PfTR Chimeric Mutants with Trx, Selenocystine, and Lipoic Acid.

		mol of NADPH min^–1 (mol of TR)⁻¹
enzyme	PfTR	90 μM Trx	300 μM selenocystine	5 mM lipoic acid	50 mM H₂O₂
PfTR-GC₁GGGKC₂G^a	1	500 ± 20	410 ± 30	90 ± 10	9 ± 0.2
PfTR-GC₁C₂G^a	11	1.3 ± 0.6	280 ± 15	70 ± 2	ND^c
PfTR-GC₁U₂G^b	12	1160 ± 20	2130 ± 60	255 ± 10	330 ± 3
PfTR-GC₁GGGKU₂G^b	13	85 ± 5	260 ± 15	145 ± 5	60 ± 3

^a

Recombinant protein produced as a GST fusion.

^b

Recombinant protein produced as an intein fusion via semisynthesis.

^c

No activity detectable.