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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Feb 10;192(6):2576–2584. doi: 10.4049/jimmunol.1301857

Figure 1. Kinetics of SD-4 or DC-HIL expression in T cells or myeloid cells during induction of EAE.

Figure 1

Spleen or draining LN cells were prepared from WT mice at different time points (days) after EAE-immunization and examined by flow cytometry for frequency (%) of SD-4+ or PD-1+ cells in the total number of CD4+ (A) or CD8+ T cells (B). Data (mean ± sd, n=3) are plotted on time points. (C and D) Similar preparations from spleens were also examined for frequency of CD11b+Gr-1+ MDSC, F4/80+ macrophages (MΦ), or CD11c+ DC (C), and for proportion of DC-HIL+ cells in each myeloid population (D). (E) Fourteen days post-EAE-immunization, CNS-infiltrating cells were isolated and assayed for frequency of CD11b+Gr1+ cells, CD4+, and CD8+ T cells, in which DC-HIL or SD-4 expression was measured. Data shown are representative of 3 independent experiments.