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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Feb 10;192(6):2576–2584. doi: 10.4049/jimmunol.1301857

Figure 5. Among DC-HIL-expressing myeloid cells in EAE-developing mice, MDSC are the most potent suppressors.

Figure 5

(A and B) Three myeloid cell subsets were purified from spleen of WT (A) or DC-HIL KO (B) mice that were EAE-immunized 10 days prior. These cells were assayed separately for T cell-suppressive activity by coculturing with CFSE-labeled WT-T cells at varying cell ratios in the presence of anti-CD3/CD28 Ab. Three days after culture, % of proliferated cells was examined by flow cytometry and data are shown in histograms. (C) Similar assays were performed, but with unlabeled T cells. T cell activation is shown by IL-2 production (mean ± sd, n=3). “None” means T cells and Ab without myeloid cells. *p<0.001 between WT and KO mice. Data are representative of 3 independent experiments.