Extended Data Figure 9. Early embryonic lethality and dysregulation of imprinted gene expression in Tet1 paternal KO mice generated by Dawlaty et al.

a, Representative image of PatKO pups from the same litter showing big variation in body and placenta sizes. Embryos and their placentae were recovered by C-section at the day of birth (E19.5). b, Average placental weights at E19.5. Error bars indicate S.E.M. **P<0.01. c, Mating scores of xHet (Tet1+/− male × wild-type female) and xHomo (Tet1−/− male × wild-type female). Black dots indicate the number of pups for each litter recovered by C-section at E19.5. Red lines indicate the mean litter size. d, Representative image of E10.5 Tet1-PatKO embryos from the mating of wild-type female with Tet1-KO male. Dotted circle indicates morphologically abnormal embryos. e, Left, E9.5 PatKO embryos recovered from a single litter. Dotted circles indicate small embryos whose placentae are morphologically abnormal. Right, placentae of the corresponding embryos showing at the left panel. Red rectangles indicate morphologically abnormal placentae. f, Summary of embryonic abnormality. * The numbers of morphologically abnormal embryos (E10.5) or placentae (E9.5). g, RT-qPCR analysis of selected imprinted genes in each of the E9.5 embryos from two litters (PatKO-1 and 2) are shown. Arrows indicate the samples showing marked change in expression level. The Ct values are normalized to the expression level of Gapdh.