Figure 6.

Hyperthermia causes DNA and RNA damage in Tau-deficient neurons. Sagittal hippocampus sections from KO-Tau mice subjected to HS were pre-treated or not with DNAse-free RNAse, RNAse-free DNAse or both before TUNEL staining and imaged using laser scanning confocal microscopy. RNAse pre-treatment fully abolished cytoplasmic staining and reduced nuclear TUNEL staining levels. DNAse pre-treatment fully abolished DAPI staining and reduced nuclear TUNEL staining. These data demonstrated that Tau deficiency induced nuclear DNA and cytoplasmic/nuclear RNA damage under HS.