Table 3.

Functional analysis of actual versus random miRNAs

	GO molecular function class
	Transcription regulator	Apoptosis regulator	Cell adhesion molecule	Binding	Enzyme	Transporter	Signal transducer	Translation regulator	Motor	Enzyme regulator	Structural molecule	Chaperone
Actual miRNAs	113	6	10	146	128	35	66	4	6	9	6	1
Random miRNAs (average)	43	1	2	98	88	22	41	2	4	9	7	1
Standard deviation	± 7.5	± 0.9	± 1.5	± 13.3	± 13.7	± 4.9	± 9.7	± 1.3	± 2.2	± 3.9	± 2.6	± 1.2
Z-score	9.3	5.9	5.0	3.6	2.9	2.7	2.6	1.4	1.0	0.01	-0.3	-0.3

Integers are number of detected conserved cases in each class. The standard deviation is for 50 experiments. The Z-scores for seven functional classes indicate over-represention for actual miRNA target genes. The thresholds used are S: 100; ΔG: -19 kcal/mol; ID: 70%.