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. 2014 Apr 1;141(7):1442–1452. doi: 10.1242/dev.103283

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© 2014. Published by The Company of Biologists Ltd

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Fig. 10. — Osr1 maintains nephron progenitor cells by inhibiting β-catenin/TCF-mediated activation of Wnt4 expression. (A,B) X-gal staining of frozen sections of E12.5 Osr1^c/+;Six2^GCE/+;Wnt4e-lacZ^tg (A) and Osr1^c/c;Six2^GCE/+;Wnt4e-lacZ^tg (B) embryos. Black dashes outline UBs. (C,D) Higher magnification views of kidney sections showing restricted lacZ expression in the renal vesicles in Osr1^c/+;Six2^GCE/+;Wnt4e-lacZ^tg control (C) and ectopic lacZ expression in the cap mesenchyme in Osr1^c/+;Six2^GCE/+;Wnt4e-lacZ^tg mutant (D) kidneys. Scale bars: 50 μm.