Fig. 3.

Aberrant accumulation of APC/C substrates required for cell cycle progression does not lead to the necrosis of neuroblasts in fzy⁵⁰³² brains. (A-F) Overexpression of non-degradable known APC/C substrates required for cell cycle progression leads to mitotic catastrophe in neuroblasts. Brains from control and fzy⁵⁰³² larvae were stained for Lamin (Lam) and with Hoechst. Worniu-Gal4 (Wor) allows neuroblast-specific overexpression of the UAS transgene. cycA^Δ170 encodes non-degradable Cyclin A. The hs-cycB^S transgene allows overexpression of non-degradable Cyclin B induced by a heat-shock promoter. pim^dba encodes non-degradable Pim. Neuroblasts are outlined in yellow. Scale bar: 10 μm. (F) The average number of neuroblasts per brain lobe is shown. (G) Aberrant accumulation of toxic proteins or blocking proteasome function is not sufficient to induce neuroblast loss. TDP43^Δnls encodes a mutant form of TDP43. VCP^R152H encodes a mutant form of VCP. pros26B and prosβ encode dominant-negative forms of 26S proteasome components. The average number of neuroblasts per brain lobe is shown.