Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 1;141(7):1492–1502. doi: 10.1242/dev.098954

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 6. — Hox4 proteins are necessary and sufficient to induce non-autonomous apical cell enlargement. (A-H) Confocal immunostaining for Hoxb4 (green) and ZO-1 (red) on E10.5 flat-mounted mouse hindbrains of wild-type (Hoxb4^+/+; Hoxd4^+/+) (A,B), Hoxb4^+/+; Hoxd4^-/- (D), Hoxb4^+/-; Hoxd4^-/- (F) and Hox4 double-mutant (H,I) embryos at a lateral level of the r6/r7 boundary region. The r6/r7 boundary or its presumptive position (x) are indicated in A and H. (B,I) Confocal z-projections of similar magnifications of the r6/r7 boundary region as in D (corresponding to dotted boxes in A and H) showing the apical cell outlines (yellow) used for quantitation. (C,E,G,J) Apical areas (μm²) with respect to the anteroposterior position of the cells in B,D,F,I, respectively. Hoxb4⁺ (green) and Hoxb4^- (black) cells are indicated. (K,L) Confocal z-projections and ZO-1 apical outlines (yellow) of the chick r5 neuroepithelium electroporated with mouse Hoxb4-mGFP. (K) Mouse Hoxb4/GFP⁺ cells (green) form a cluster with a well-defined interface (dotted line). (L) Corresponding apical cell areas (μm²) with respect to anteroposterior position for mouse Hoxb4⁺ (green) and mouse Hoxb4^- (black) cells. Anterior is towards the left.