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. 2014 Apr 1;141(7):1514–1525. doi: 10.1242/dev.102426

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Small cells with large vesicles containing secretory material. (A) Small cells (yellow circles) are not labelled by markers for ciliated cells (anti-AcTub), ionocytes (anti-V1a) or goblet cells (anti-Xeel). Membranes are marked with membrane GFP (mGFP). (B) SEM shows small cells with large apical openings and secretory material highlighted with an arrow. Sections imaged by TEM show vesicles at the apical membrane containing a dark core surrounded by lighter material. Highlighted with a yellow arrow is a vesicle deeper within the cytoplasm that may represent an immature vesicle. Scale bars: in A, 10 μm; in B, 2 μm (SEM), 1 μm (TEM, low magnification) and 0.5 μm (TEM, high magnification).