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. 2014 Apr 1;141(7):1514–1525. doi: 10.1242/dev.102426

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Molecular profile of the new cell type. (A) In situ hybridisation using an antisense probe to the itpkb transcript reveals a scattered, punctate epidermal pattern. (B) Double fluorescence in situ hybridisation for foxa1 and itpkb shows colocalisation of the two transcripts. (C) Antibody staining for Itpkb with mGFP shows localisation in the small cells with large apical vesicles. (D) PNA conjugated to Alexa Fluor-568 colocalises with foxa1. (E) PNA together with mGFP staining shows strong labelling of material in the large vesicles. Staining is also evident at a lower level in vesicles of goblet cells (yellow arrow). (F) Antibody staining to the carbohydrate epitope HNK-1 together with mGFP shows specific expression in the vesicles of the small cells. (G) Co-staining for HNK-1 and PNA shows that they are present in the same cell. Scale bars: 500 μm in A; 50 μm in B; 5 μm in C,E-G; 15 μm in D.