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. 2014 Apr 1;141(7):1514–1525. doi: 10.1242/dev.102426

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — Impact of otogelin-like knockdown on the epidermis. (A) The otogelin-like gene is predicted to have up to 79 exons and 78 introns. An antisense morpholino oligo (red) was designed against the splice junction of exon 1 and intron 1. Loss of otogelin-like transcript is evident with otogelin-like MO compared with controls (MOC) by RT-PCR, indicating knockdown of expression. There is little difference in the intensity of bands for the ubiquitously expressed gene, ornithine decarboxylase (ODC). (B) Comparison of PNA staining in otogelin-like morphants compared with controls (MOC). Right panels are higher magnification images of the flank epidermis. (C) TEM images of goblet cells and SSCs in controls (MOC) and otog-like MO treated embryos. Note absence of dark core in the vesicles of the SSCs. Scale bars: in B, 250 μm (left panels) and 50 μm (right panels); in C, 1 μm.