Figure 1.

Retinal flat mount of mouse eye with oxygen-induced retinopathy showing blood vessels after perfusion with fluorescein isothiocyanate (FITC) coupled to high-molecular-weight dextran. The retina was divided into three concentric zones: the inner zone around the optic disc (A), the middle zone (B), and the outer zone (C) for scoring of avascular areas on a central—peripheral axis. Vascular proliferations were quantified by counting blood vessel tufts and cluster formation (presumed extra-retinal neovascularization) in each of 12 equally sized sections of the retina (numbers). Bar = 1 μm.