Figure 3.

Invariant chain (Ii) mRNA detected in gastric epithelial cells by RT-PCR analysis. RNA extracted from KATO III and N87 gastric epithelial cells was reverse transcribed, amplified, and separated by electrophoresis. Specific products were detected in these cells by RT-PCR using the Ii chain primers described in Materials and Methods. As an internal control, B (Jesthom) cells showed the mRNA for those proteins. The amplification of housekeeping gene GAPDH was included as a control for the procedure. This is a representative of three experiments.