NIM-1 efficiently detects demyelination in a cuprizone-induced animal demyelination model. Brain coronal sections from control mice (A, C, E) and cuprizone-fed mice (B, D, F) were stained with Luxol Fast Blue (A, B), MBP (C, D), and NIM-1 dye (E, F). Shown are middle portions of CC. Bars: A-D = 100 μm; E-F = 200 μm.