Figure 1.

Specificity of the different CCK_A and CCK_B receptor antibodies established by Western blotting. Rat pancreatic membranes (30 μg) were used for Western blotting analysis with antibodies 1122 (1:10,000), AR6 (1:1000), R1-2 (1:1000), 9262 (1:10,000), 9491 (1:1000), and GR4 (1:10,000). Specificity was established by preincubation of each primary antibody for 2 hr at RT with 40 μg/mL⁻¹ of the corresponding peptide antigen, with the exception of antibody GR4 for which the peptide antigen was unavailable. Ab, antibody; P, peptide antigen.