FIG 3.

RT-qPCR confirms overexpression of EhCoactosin and SREHP. RNA from untransfected (wild type; WT) log-phase E. histolytica trophozoites or from trophozoites transfected with an expression vector encoding SREHP or EhCoactosin was used for RT-qPCR analysis of expression. The ssRNA gene was used as a loading control. SREHP and EhCoactosin were expressed at approximately 3.6 (±0.5)-fold and 7.1 (±1.8)-fold, respectively, above wild-type levels. The data represent the means ± SD from 2 biological replicates and 3 technical replicates per biological replicate (**, P < 0.01; ***, P < 0.001).