FIG 4.

SREHP and EhCoactosin overexpressors are less susceptible to wortmannin and LY294002 toxicity. Transgenic trophozoites were exposed to 1 mM or 2 mM wortmannin (A), 50 μM LY294002 (B), or 25 μM LY294002 (C) for 3 days, after which viability was assessed. The data are reported as a percentage of diluent-treated control amoebae and represent the means ± SD from 3 trials (*, P < 0.05). (A) SREHP- and EhCoactosin-overexpressing cells exhibited increased survival in the presence of wortmannin compared to untransfected wild-type (WT) cells or another transgenic cell line overexpressing an irrelevant gene, EhLimA, from the same expression vector. (B) SREHP-overexpressing cells exhibited enhanced survival in the presence of 50 μM LY294002. (C) EhCoactosin-overexpressing cells exhibited enhanced survival in the presence of 25 μM LY204002.