FIG 8.
GFP-PHBtk overexpressors possess increased levels of EhCoactosin and SREHP transcripts. RNA from untransfected (wild type; WT) log-phase E. histolytica trophozoites, from a cell line expressing a GFP-tagged PH domain (GFP-PHBtk), and from a cell line expressing an irrelevant protein, luciferase (Eh209), was used for qPCR analysis of expression. The ssRNA gene was used as a loading control. (A) SREHP was expressed in the GFP-PHBtk cell line at a level that was approximately 1.65 (±0.07)-fold (**, P < 0.01) higher than that in WT cells. (B) EhCoactosin was expressed in the GFP-PHBtk cell line at a level that was approximately 3.4 (±0.57)-fold (***, P < 0.001) higher than that in WT levels cells. Neither SREHP nor EhCoactosin was expressed at a higher level in the control cell line (Eh209), suggesting that the increase seen in the GFP-PHBtk cell line was authentic. The data represent the means ± SD from ≥3 biological replicates.